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Cet atlas de poche est un grand classique. Pratique et exhaustif, il réussit un véritable pari pédagogique : celui de rendre accessibles, de façon particulièrement attrayante, des notions d'histologie souvent complexes et arides. Il guide le lecteur de façon claire et didactique dans la description des différents types cellulaires, du noyau de la cellule, du cytoplasme, de tous les organites intra-cellulaires, dans la description des principaux tissus et dans la compréhension des mécanismes de régulation. Cette nouvelle édition comporte 745 illustrations en couleurs d'histologie et de microscopie électronique d'une qualité exceptionnelle, accompagnées pour chacune, d'une légende, d'un texte explicatif bref et concis et de renvois à d'autres illustrations. De nombreux tableaux synoptiques de diagnostic différentiel permettent une identification rapide des préparations grâce aux caractéristiques spécifiques. La juxtaposition particulière texte-photo, la richesse et la clarté des tableaux en annexe, l'index très détaillé font de ce classique un outil précieux et indispensable pour préparer, réviser et réussir l'examen d'histologie. Ce livre s'adresse aux étudiants en médecine, en biologie, en odontologie et en médecine vétérinaire, mais il intéressera également les anatomo-pathologistes, les biologistes et les médecins soucieux d'actualiser leurs connaissances en histologie moderne. ›

Histology --- Cell Biology --- Atlas

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Directed evolution, the application of evolutionary design to enzyme engineering, requires effective screening strategies to isolate those proteins that perform a desired function from the libraries generated by the techniques. In Directed Enzyme Evolution: Screening and Selection Methods, seasoned practitioners from many leading laboratories describe their leading and readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes. The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens. There are also representative examples of how phage libraries may be interrogated for enzymatic activity. Each protocol contains detailed step-by-step instructions and many notes on how best to deal with the problems that may occur. An accompanying volume, Directed Evolution Library Creation: Methods and Protocols (ISBN 1-58829-285-1), describes readily reproducible methods for the creation of mutated DNA molecules and DNA libraries. Taken together, Directed Enzyme Evolution: Screening and Selection Methods and Directed Evolution Library Creation: Methods and Protocols capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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With the decoding of the human genome and the potential application of its information to gene chips, the ability to generate cDNA libraries for functional assays of specific gene sequences has become critical in gene manipulation and genetic research. In Generation of cDNA Libraries: Methods and Protocols, expert researchers and inventors in the field describe their own proven techniques for generating cDNA/mRNA libraries to identify the functions of specific decoded gene sequences. A wide variety of techniques is presented for enhancing the generation of complete and full-length libraries, and for confirming the quality of the cDNAs generated. Among the applications detailed are electrophoresis, Northern blotting, single-cell microarray analysis, subtractive hybridization, subtractive cloning, gene cloning, and peptide library generation. Each method includes background information, step-by-step instructions, a list of reagents, operational tips, and notes on instrumentation. A background tutorial provides essential information: the definition of a cDNA library, the various types of cDNA libraries, the differences between methods for cDNA library generation using either conventional approaches or novel strategies, and the quality of cDNA libraries. State-of-the-art and highly practical, Generation of cDNA Libraries: Methods and Protocols provides a variety of powerful and readily reproducible techniques for the generation of the entire range of complete, full-length cDNA/mRNA libraries needed in today's forefront genetic research.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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Directed protein evolution-a powerful technique for the discovery of new enzymes and therapeutic proteins-has matured to the point that it is now the centerpiece of enzyme engineering, where it has catalyzed the development of numerous experimental methods. In Directed Evolution Library Creation: Methods and Protocols, seasoned practitioners from many leading laboratories share their best experimental protocols for the generation of molecular diversity. Described in step-by-step detail to ensure experimental success, these protocols include readily reproducible methods for random mutagenesis of entire genes or segments of genes, for homologous and nonhomologous recombination, and for constructing in vivo libraries in bacteria and yeast. In addition to the various protocols for creating libraries, this volume also describes ways to analyze libraries, particularly those made by recombination. An accompanying volume, Directed Enzyme Evolution: Screening and Selection Methods (ISBN: 1-58829-286-X), is devoted entirely to selection and screening methods that can be applied to the directed evolution of enzymes. Taken together, Directed Evolution Library Creation: Methods and Protocols and Directed Enzyme Evolution: Screening and Selection Methods capture for newcomers and more experienced investigators alike all the key methods for using directed protein evolution to better understand protein structure-function relationships, to discover new enzymes and therapeutic proteins, and to design new assays suitable for specific applications.

Cytology. --- Cell Biology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Cytologists

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The acclaimed International Review of Cytology series presents current advances and reviews in cell biology, both plant and animal. Aricles address structure and control of gene expression, nucleocytoplasmic interactions, control of cell development and differentiation, and cell transformation and growth. Contributors to this volume include Yosef Gruenbaum, Sergey Razin, Johanna M. van der Wouden, J. M. Mitchison, Ora A. Weisz, andAnne Regnier-Vigourous.The acclaimed International Review of Cytology series presents current advances and reviews in cell biology, both pl

Cells. --- Cytology. --- Cell biology --- Cellular biology --- Biology --- Cells --- Organisms --- Cytology